General Publications

High density Cell Culture in a new passive membrane based bioreactor

M. Wolf, T. DeSutter; Biotechnology
International 1999

Celline Overview, Application Introduction

This report is an overview of the CELLine regarding its operation and the principle of cell compartment viable cell capacity. This report describes the impact of nutrient medium exchange and the diffusion of solute across the semi permeable membrane. The importance of inoculation density and its impact on culture initiation is described. The variable volume of the cell compartment, expected osmotic water flux, and equivalency between the different CELLine models is also reported. >more

M. Wolf; Wilson Wolf Corporation,
Minneapolis USA

Antibody Expression in Hybridoma

Dialysis-based bioreactor systems for the production of mono-clonal antibodies - alternatives to ascites production in mice

M.P. Bruce, V. Boyd, C. Duch, J.R. White; Journal of Immunological Methods 2002, 264: 59-68

Manufacture of Pure Monoclonal Antibodies by Heterogeneous Culture without Downstream Purification

L.E. Scott, H. Aggett,
D.K. Glencross; Biotechniques 2001, 31-3: 666-68

Efficient laboratory-scale production of monoclonal antibodies using membrane-based high density cell culture technology
>more

M. Trebak, J.M. Chong, D. Herlyn, D.W. Speicher; Journal of Immunological
Methods 1999, 230: 59-70

Comparison of batch vs. Celline culture for production of mono-clonal antibody in vitro as alternatives to ascites. Application: Murine Hybridoma

This report is a comparison of antibody production in the CELLine relative to a traditional batch culture method. The seven hybridoma clones which were cultured in the CELLine had been previously cultured using a traditional batch method. Comparison of results obtained in the CELLine and in batch culture is provided. The benefits of a concentrated product, reduced handling, and reduced serum use are demonstrated. The consumption of nutrient medium by the two different methods is reported and provided for each clone.>more

M. Wolf; Wilson Wolf Corporation,
Minneapolis USA

Antibody Manufacture in the Celline CL1000, Application: Murine hybridoma

This report includes results from the culture of over 30 different hybridoma clones in the CELLine. The results from the large number of different clones provides a range of performance which the user can use to compare their individual results. Mean target values for cell numbers, antibody yield and harvest times and volume are provided derived from cultures of different hybridoma clones. Importantly, the clones were randomly selected representing numerous mAb isotypes and fusion partners. Additionally, all the cultures were carried out in a small manufacturing environment aimed towards reducing overall costs and labor.>more

M. Wolf; Wilson Wolf Corporation,
Minneapolis USA

Attachment dependent cell cultivation on Micro-Carriers: Secreted protein production >more

M. Wolf; Wilson Wolf Corporation,
Minneapolis USA

Membrane-based cell culture systems - an alternative to in Vivo production of monoclonal antibodies

A new generation of membrane based cell culture devices tailored to small scale production of monoclonal antibodies (mab´s) entered the market in last few years. In contrast to conventional perfusion hollow fiber bioreactors, these devices contain two functionally different membranes - one dialysis membrane for nutrient supply and one gas permeable membrane for direct oxygenation of cells. The latest systems of this generation are static culture systems of moderate costs and either better than, or equal to, the ascites mice in terms of quality and quantity of produced monoclonal antibodies. We have investigated the advantages of the perfused Tecnomouse bioreactor and the static CELLine culture flasks in comparison to ascites production and conventional roller bottle cultures.>more

A. Nagel, S. Koch, U. Valley,
F. Emmrich, U. Marx; Dev Biol Stand 1999, 101: 57-64

Rapid evaluation of hybridoma behaviour in high cell density pro-duction process

S. Koch, A. Kriszo, C. Kloth, U. Marx, A. Nagel; ESACT 1999, Lugano 1999

Membrane-based cell culture technologies: a scientifically and economically satisfactory alternative to malignant ascites production for monoclonal antibodies

U. Marx; Res Immunol 1998, 149: 557-9

Protein expression in CHO cells

A soluble TGF-b type-1 Receptor mimics TGFb responses

F. Docagne, N. Colloc'h, V. Bougueret, M. Page, J. Paput, M. Tripier, P. Dutartre,
E.T. MacKenzie, A. Buisson, S. Komesli, D. Vivien; Journal of Biological Chemistry
2001, 276-49: 46243-50

High density suspension culture for Recombinant Protein produc-tion from CHO cells >more

M. Wolf; Wilson Wolf Corporation,
Minneapolis USA

Protein expression in BHK cells

Long-Term High Level Protein Expression in Adherent, Protein-free Growing BHK Cells Using INTEGRA CELLine adhere 1000 Bio-reactor Flasks

J. Mittermaier, M. O. Zang-Gandor; EUGENEX Biotechnologies GmbH,
Tägerwilen, Switzerland

Protein expression in 293 cells

Improved Method of Utilizing Transient Transfections in HEK293E Cells for Small Scale Antibody Production

Masahisa Handa, Eddie Bautista, Peter Wong, Robyn Cotter, Lynn Webster, Arnold Horwitz

Mulit-Milligram Production of Antibodies Using Transiently Transfected HEK293 Cells Cultured in Integra Flasks

Eddie Bautista, Masahisa Handa

Production in Baculovirus Infected SF9 Cells

Continuous Recombinant Protein Production in Baculovirus Infected SF9 Cells using CELLine classic 1000 Two-Compartment Bioreactors

Izumi Matsumoto (GSI Creos GmbH, Tokio, Japan) and Alex Studer (INTEGRA Biosciences, Chur, Switzerland)

Fermentation

The Application of in vitro Models for Production of Metabolites: Isolation and Characterisation of Hydroxysecobarbital

D. Marshall, M. Robinson, P. Hincks, M. Dumasia, P. Teale, E. Houghton; hromatographia
Supplement 2000, 52: 35-38

Protein production in plant cells

Production of Human alpha-1-Antitrypsin from Transgenic Rice Cell Culture in a Membrane Bioreactor

McDonald KA et al. 2005,
21: 728-34