Wilson Wolf Manufacturing

Dialysis-based bioreactor systems for the production of mono-clonal antibodies - alternatives to ascites production in mice

• M.P. Bruce, V. Boyd, C. Duch, J.R. White; Journal of Immunological Methods 2002, 264: 59-68

Manufacture of Pure Monoclonal Antibodies by Heterogeneous Culture without Downstream Purification

• L.E. Scott, H. Aggett, D.K. Glencross; Biotechniques 2001, 31-3: 666-68

Comparison of batch vs. Celline culture for production of mono-clonal antibody in vitro as alternatives to ascites. Application: Murine Hybridoma

• M. Wolf; Wilson Wolf Corporation, Minneapolis USA

This report is a comparison of antibody production in the CELLine relative to a traditional batch culture method. The seven hybridoma clones which were cultured in the CELLine had been previously cultured using a traditional batch method. Comparison of results obtained in the CELLine and in batch culture is provided. The benefits of a concentrated product, reduced handling, and reduced serum use are demonstrated. The consumption of nutrient medium by the two different methods is reported and provided for each clone.

Membrane-based cell culture systems - an alternative to in Vivo production of monoclonal antibodies

• A. Nagel, S. Koch, U. Valley, F. Emmrich, U. Marx; Dev Biol Stand 1999, 101: 57-64

A new generation of membrane based cell culture devices tailored to small scale production of monoclonal antibodies (mab´s) entered the market in last few years. In contrast to conventional perfusion hollow fiber bioreactors, these devices contain two functionally different membranes - one dialysis membrane for nutrient supply and one gas permeable membrane for direct oxygenation of cells. The latest systems of this generation are static culture systems of moderate costs and either better than, or equal to, the ascites mice in terms of quality and quantity of produced monoclonal antibodies. We have investigated the advantages of the perfused Tecnomouse bioreactor and the static CELLine culture flasks in comparison to ascites production and conventional roller bottle cultures.

Rapid evaluation of hybridoma behaviour in high cell density pro-duction process

• S. Koch, A. Kriszo, C. Kloth, U. Marx, A. Nagel; ESACT 1999, Lugano 1999

Membrane-based cell culture technologies: a scientifically and economically satisfactory alternative to malignant ascites production for monoclonal antibodies

• U. Marx; Res Immunol 1998, 149: 557-9