Provides data which demonstrates G-Rex supporting higher fold cell expansion of NK cells compared to conventional gas-permeable bags.
Large-Scale Culture and Genetic Modification of Human Natural Killer Cells for Cellular Therapy
Authors: Natalia Lapteva, et al
Publication: Natural Killer Cells: Methods and Protocols, Methods in Molecular Biology
Published Date : 7/1/2016
Recent advances in methods for the ex vivo expansion of human natural killer (NK) cells have facilitated the use of these powerful immune cells in clinical protocols. Authors provide details on their successful adaptation of an expansion method for primary NK cells from peripheral blood mononuclear cells or from apheresis products in gas permeable rapid expansion devices (G-Rex devices).
Demonstrates how G-Rex overcomes the traditional obstacle to culturing TIL cells by supporting increased expansion while maintaining clonal diversity and increased mitochondrial function.
The beneficial effects of a gas-permeable flask for expansion of Tumor-Infiltrating lymphocytes as reflected in their mitochondrial function and respiration capacity
Authors: Marie-Andrée Forget, et al
Publication: OncoImmunology
Published Date : 3/2/2016
Adoptive transfer of autologous ex vivo expanded tumor-infiltrating lymphocytes (TIL) is a highly successful cell therapy approach in the treatment of late-stage melanoma. To make this therapy broadly available, one of the major obstacles to overcome is the complexity of culturing the TIL. The use of the Gas permeable flask (G-Rex) during the REP has been one application that has facilitated this process. Here we show that the use of this new device is able to rescue poor TIL growth and maintain clonal diversity while supporting an improved mitochondrial function.
Demonstrates capabilities of G-Rex to support a production scale necessary to manufacture TIL for the treatment of melanoma.
Activation and Propagation of Tumor-infiltrating Lymphocytes on Clinical-grade Designer Artificial Antigen-presenting Cells for Adoptive Immunotherapy of Melanoma
Authors: Marie-Andree Forget, et al
Publication: Immunotherapy Journal
Published Date : 11/1/2014
Adoptive cell therapy with autologous tumor-infiltrating lymphocytes (TIL) is a therapy for metastatic melanoma with response rates of up to 50%. However, the generation of the TIL
transfer product is challenging, requiring pooled allogeneic normal donor peripheral blood mononuclear cells (PBMC) used in vitro as “feeders” to support a rapid-expansion protocol. Here, we optimized a platform to propagate TIL to a clinical scale using K562 cells genetically modified to express costimulatory molecules such as CD86, CD137-ligand, and membrane-bound IL-15 to function as artificial antigen-presenting cells (aAPC) as an alternative to
using PBMC feeders.
Provides a comprehensive overview of the underlying principles that make G-Rex M the most efficient and versatile style of G-Rex device.
Optimizing the production of suspension cells using the G-Rex “M” series
Authors: Pradip Bajgain, et al
Publication: Molecular Therapy — Methods & Clinical Development
Published Date : 11/1/2014
Broader implementation of cell-based therapies has been hindered by the logistics associated with the expansion of clinically relevant cell numbers ex vivo. To overcome this limitation, Wilson Wolf Manufacturing developed the G-Rex, a cell culture flask with a gas-permeable membrane at the base that supports large media volumes without compromising gas exchange. In this study, we investigated multiple variables including optimal seeding density and media volume, as well as maximum cell output per unit of surface area. A multicenter study confirmed that this fully optimized cell culture system can reliably produce a 100-fold cell expansion in only 10 days using 1L of medium.
Demonstrates how G-Rex both simplifies and improves the production of TIL cells compared to conventional methods.
Simplified Method of the Growth of Human Tumor Infiltrating Lymphocytes in Gas-permeable Flasks to Numbers Needed for Patient Treatment
Authors: Jianjian Jin, et al
Publication: Journal of Immunotherapy
Published Date : 4/1/2012
Adoptive cell therapy of metastatic melanoma with autologous tumor infiltrating lymphocytes (TIL) is clinically effective, but TIL production can be challenging. Here we describe a simplified method for initial TIL culture and rapid expansion in gas-permeable flasks.
Provides details on the underlying principles that make G-Rex the best T-cell production technology.
Accelerated Production of Antigen-specific T Cells for Preclinical and Clinical Applications Using Gas-permeable Rapid Expansion Cultureware
Authors: Juan F. Vera, et al
Publication: Immunotherapy Journal
Published Date : 4/1/2010
Provides data which demonstrate why G-Rex is the best culture technology for production of antigen-specific T cells. It provides extensive scientific analysis of how to optimize culture conditions in G-Rex to provide many benefits.