Notable Quotes:

“With a starting density of 5x10e6 cells per GREX well, we achieved a median density of 47.1x19e6 cells per GREX well”

“This outcome, with maintenance of a high central memory population, is an improvement over the primarily effector memory population produced using a longer culture time when rhesus macque T cells were transduced with a gammaretrovirus to express CXCR5”

Notable Quotes:

“Showed sufficient transduction efficiency (day7) with low MOI in scaled down model for transduction in G-Rex”

“The platform includes pre-evaluated and qualified equipment, technologies, and materials with developed closed unit operations. “

“To enable swift onboarding of the customer process through manufacturing, WuXi has established template batch records, bill of materials, standardized testing catalog, and specifications for cGMP materials.”

Notable Quotes:

“This study demonstrates that T cells can be transduced with retroviral vectors in solution in the G-Rex bioreactor with addition of Vectofusin-1 as a transduction enhancer. The simplified transduction step allows for an entire process, from activation to harvest, to be carried out in a single vessel. “

“Clinically relevant levels of transgene expression and cell numbers can be achieved by combining reagents in the GRex, without complicated time-consuming coating steps of traditional transduction.”

First publication detailing transduction taking place in G-Rex. Additionally, they compared the All-In-One G-Rex process to the traditional method of CAR T production in gas permeable bags. “Further, the G-Rex based process reduced the cost of materials by 38% and hand-on time by 75% to generate a batch of 1.4x10e9 transgenic cells.” There is an excellent table displaying the cost reduction and labor savings.

Notable Quotes:

Average for all samples was 18.9 billion TCR transduced T cells per one G-Rex500M-CS. “This study used a single G-REX culture vessel to obtain approximately 20x10e9 T-cells, but the cell yield can easily be increased further by using multiple vessels.”

“G-REX, with fivefold greater volume and when these larger vessels are used, media and cells can be added and removed without opening the system. Earlier renditions of this protocol and other protocols similar to this, which yielded ~ 20–30 × 109 cells, required up to 40 T-175 flasks during the first 7 days of the REP.”

Provides data which demonstrate why G-Rex is the best culture technology for production of antigen-specific T cells. It provides extensive scientific analysis of how to optimize culture conditions in G-Rex to provide many benefits.