Provides details on using G-Rex for an efficient approach to generate highly functional Vγ9Vδ2 T cells in massive numbers suitable for clinical application in an allogeneic setting.
Large-scale expansion ofVγ9Vδ2T cells with engineered K562 feeder cells in G-Rex vessels and their use as chimeric antigen receptor– modified effector cells
Authors: Lin Xiao, et al
Publication: Cytotherapy
Published Date : 12/17/2017
Notable Quotes:

“optimized the co-culture ratio of K562 aAPCs to immune cells, and migrated this method to a G-Rex cell growth platform to derive clinically relevant cell numbers in a Good Manufacturing Practice (GMP)-compliant manner”
Clinically relevant numbers of NK cells from unseparated apheresis or PBMCs in 10 days. Methods Chapter describing Baylor College of Medicine producing clinically relevant numbers of NK cells from unseparated apheresis or PBMCs in 10 days using G-Rex. Describing the methodologies as GMP compliant and suitable for use in clinical-grade product manufacturing.
Large-Scale Culture and Genetic Modification of Human Natural Killer Cells for Cellular Therapy
Authors: Natalia Lapteva, et al
Publication: Methods in Microbiology
Published Date : 1/1/2016
Notable Quotes:

“Cultures in G-Rex (optimal 10 mL of medium per cm2 of surface area of a gas-permeable membrane) typically require no cell manipulation or feeding during the 10 days of culture period.”
Expansion of Human and Murine Hematopoietic Stem and Progenitor Cells Ex Vivo without Genetic Modification Using MYC and Bcl-2 Fusion Proteins
Authors: Gregory A. Bird, et al
Publication: PLOS One
Published Date : 8/1/2014