Review of a serum-free method of ex vivo CIK cell expansion based on the use of G-Rex static bioreactors, which drastically reduces culture manipulations and can be easily reproduced under GMP conditions as well as scaled for larger cell numbers.
A serum-free protocol for the ex vivo expansion of Cytokine-Induced Killer cells using gas-permeable static culture flasks
Authors: Palmerini, et al
Publication: Cytotherapy
Published Date : 9/1/2020
Notable Quotes:

“G-Rex led to large numbers of CIK cells, with a minimal need for technical interventions, thus reducing the time and costs of culture manipulation....The described procedure can be easily translated to large-scale production under Good Manufacturing Practice.”

“Thus, the higher proportion of naïve and central memory CD3+CD56-T cells in the final CIK cell product, which we demonstrate to occur in the G-Rex cultures, could ensure a source of new post-infusion CIK effector cells that may possibly contribute to higher survival rates and long-term clinical responses.”
Provides details on using G-Rex for an efficient approach to generate highly functional Vγ9Vδ2 T cells in massive numbers suitable for clinical application in an allogeneic setting.
Large-scale expansion ofVγ9Vδ2T cells with engineered K562 feeder cells in G-Rex vessels and their use as chimeric antigen receptor– modified effector cells
Authors: Lin Xiao, et al
Publication: Cytotherapy
Published Date : 12/17/2017
Notable Quotes:

“optimized the co-culture ratio of K562 aAPCs to immune cells, and migrated this method to a G-Rex cell growth platform to derive clinically relevant cell numbers in a Good Manufacturing Practice (GMP)-compliant manner”
Clinically relevant numbers of NK cells from unseparated apheresis or PBMCs in 10 days. Methods Chapter describing Baylor College of Medicine producing clinically relevant numbers of NK cells from unseparated apheresis or PBMCs in 10 days using G-Rex. Describing the methodologies as GMP compliant and suitable for use in clinical-grade product manufacturing.
Large-Scale Culture and Genetic Modification of Human Natural Killer Cells for Cellular Therapy
Authors: Natalia Lapteva, et al
Publication: Methods in Microbiology
Published Date : 1/1/2016
Notable Quotes:

“Cultures in G-Rex (optimal 10 mL of medium per cm2 of surface area of a gas-permeable membrane) typically require no cell manipulation or feeding during the 10 days of culture period.”